Project PN-III-P1-1_1-TE-2021-0655
New chromatographic approaches for evaluation of the antioxidant/prooxidant profile of redox-active drugs

General Information
Project code
Contract number
nr. TE 65 from 12/05/2022
Project implementation period
13.05.2022 – 12.05.2024
Project budget
450.000,00 lei
Project coordinator
Babes-Bolyai University, Cluj-Napoca, Romania
Project manager
Casoni Dorina
Project Summary
The aim of the proposed project is development and validation of a new method for in-vitro comprehensive evaluation of antioxidant/pro-oxidant profile of drugs using high-performance thin-layer chromatography combined with image processing technique (HPTLC-IA). By the proposed objectives, the performance of the chromatographic method will be improved, as the basic concept of HPTLC-IA method will be validated at laboratory level and will allow a rapid estimate of both the total antioxidant potential of drugs (using the micro-HPTLC approach) and of antioxidant/pro-oxidant profile after their interaction with different substrates (using the classical HPTLC approach). In the experimental development, chromatographic plates of different polarities (type RP-2, RP-8, RP-18, RP-18W, NH2, Diol and CN) will be used for the first time as binding substrates able to simulate the interaction of selected drugs with compounds from biological fluids and membranes and highlight key aspects of the possible influence of the physiological environment on the antioxidant potential of drugs. The chromatographic method developed and validated throughout the project will be applied in evaluation of antioxidant/prooxidant/synergist ic profile of adrenergic drugs currently considered redox-active drugs with promising applications in treatments of various diseases such as cancer and osteoporosis.
The general objective of the project
Development and validation of a new chromatographic method assisted by image analysis technique for complete evaluation of antioxidant/pro-oxidant/synergistic profile of redox-active drugs of pharmaceutical and biological interest.
Specific objectives
Objective 1
Development of a micro-HPTLC-IA protocol for accurate evaluation of the total antioxidant potential (TAP) of redox-active drugs potential (TAP)

Objective 2:
Development of a HPTLC-IA protocol for accurate evaluation of antioxidant/pro-oxidant potential of redox-active drugs after their in-vitro interaction with different substrates

Objective 3:
Evaluation of the antioxidant/pro-oxidant/synergistic potential of selected adrenergic drugs based on their radical-scavenging profile in different in-vitro simulated physiological conditions