Project PN-III-P1-1_1-TE-2021-0655
New chromatographic approaches for evaluation of the antioxidant/prooxidant profile of redox-active drugs
Objectives and activities
Objective 1
Development of a micro-HPTLC-IA protocol for accurate evaluation of the total antioxidant
potential (TAP) of redox-active drugs
Activity 1.1.
Optimization of the chromatographic conditions and image processing parameters for
the accurate evaluation of total antioxidant capacity in micro-HPTLC method for both DPPH • and
ABTS •+ protocols.
Activity 1.2.
Validation of the developed micro-HPTLC-IA method - determination of the
method performance parameters (linearity range; limit of quantification (LOQ); limit of
detection (LOD); accuracy; precision and robustness).
Objective 2
Development of a HPTLC-IA protocol for accurate evaluation of antioxidant/pro-oxidant potential of
redox-active drugs after their in-vitro interaction with different substrates
Activity 2.1.
Optimization of the chromatographic conditions and image processing
parameters for the evaluation of antioxidant capacity on different binding substrates using the
classical HPTLC method.
Activity 2.2.
Validation of the developed HPTLC-IA protocol for the evaluation of
antioxidant potential on different binding substrates.
Activity 2.3.
Evaluation of the antioxidant/prooxidant capacity of adrenergic drugs both in
aqueous medium and on simulated binding substrates.
Activity 2.4.
Evaluation of the possible synergistic effect of combinations of adrenergic
drugs with catecholamines
Objective 3
Evaluation of the antioxidant/pro-oxidant/synergistic potential of selected adrenergic drugs
based on their radical-scavenging profile in different in-vitro simulated physiological
conditions
Activity 3.1.
Evaluation of the antioxidant potential of adrenergic drugs based on the results
obtained within the developed chromatographic methods and their comparison with results from
established reference methods.