Project PN-III-P1-1_1-TE-2021-0655
New chromatographic approaches for evaluation of the antioxidant/prooxidant profile of redox-active drugs
General Information
Project code
PN-III-P1-1_1-TE-2021-0655
Contract number
nr. TE 65 from 12/05/2022
Project implementation period
13.05.2022 – 12.05.2024
Project budget
450.000,00 lei
Project coordinator
Babes-Bolyai University, Cluj-Napoca, Romania
Project manager
Casoni Dorina
Project Summary
The aim of the proposed project is development and validation of a new method for in-vitro
comprehensive evaluation of antioxidant/pro-oxidant profile of drugs using high-performance
thin-layer chromatography combined with image processing technique (HPTLC-IA). By the
proposed objectives, the performance of the chromatographic method will be improved, as the
basic concept of HPTLC-IA method will be validated at laboratory level and will allow a rapid
estimate of both the total antioxidant potential of drugs (using the micro-HPTLC approach) and
of antioxidant/pro-oxidant profile after their interaction with different substrates (using the
classical HPTLC approach). In the experimental development, chromatographic plates of
different polarities (type RP-2, RP-8, RP-18, RP-18W, NH2, Diol and CN) will be used for the
first time as binding substrates able to simulate the interaction of selected drugs with compounds
from biological fluids and membranes and highlight key aspects of the possible influence of the
physiological environment on the antioxidant potential of drugs. The chromatographic method
developed and validated throughout the project will be applied in evaluation of
antioxidant/prooxidant/synergist ic profile of adrenergic drugs currently considered redox-active
drugs with promising applications in treatments of various diseases such as cancer and
osteoporosis.
The general objective of the project
Development and validation of a new chromatographic method assisted by image analysis
technique for complete evaluation of antioxidant/pro-oxidant/synergistic profile of redox-active
drugs of pharmaceutical and biological interest.
Specific objectives
Objective 1
Development of a micro-HPTLC-IA protocol for accurate evaluation of the total antioxidant potential (TAP) of redox-active drugs potential (TAP)
Objective 2:
Development of a HPTLC-IA protocol for accurate evaluation of antioxidant/pro-oxidant potential of redox-active drugs after their in-vitro interaction with different substrates
Objective 3:
Evaluation of the antioxidant/pro-oxidant/synergistic potential of selected adrenergic drugs based on their radical-scavenging profile in different in-vitro simulated physiological conditions
Development of a micro-HPTLC-IA protocol for accurate evaluation of the total antioxidant potential (TAP) of redox-active drugs potential (TAP)
Objective 2:
Development of a HPTLC-IA protocol for accurate evaluation of antioxidant/pro-oxidant potential of redox-active drugs after their in-vitro interaction with different substrates
Objective 3:
Evaluation of the antioxidant/pro-oxidant/synergistic potential of selected adrenergic drugs based on their radical-scavenging profile in different in-vitro simulated physiological conditions